Difference between revisions of "Part:BBa K3165006:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
ECFP belongs to the family of Cyan Fluorescent proteins, where the Tyr-66 is replaced with a Trp. The ECFP coding sequence was developed by the mutations F64L, S65T, Y66W, N146I, M153T and V163A in the GFP sequence. The part was codon optimised for Escherichia coli.
+
ECFP belongs to the family of Cyan Fluorescent proteins, where the Tyr-66 is replaced with a Trp. The ECFP coding sequence was developed by the mutations F64L, S65T, Y66W, N146I, M153T and V163A in the GFP sequence. The part was codon optimised for <i>Escherichia coli</i>.
  
  

Revision as of 17:56, 18 October 2019


ECFP (Optimized for Escherichia coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ECFP belongs to the family of Cyan Fluorescent proteins, where the Tyr-66 is replaced with a Trp. The ECFP coding sequence was developed by the mutations F64L, S65T, Y66W, N146I, M153T and V163A in the GFP sequence. The part was codon optimised for Escherichia coli.


Source

This sequence is derived from the existing part BBa_E0020 in the iGEM registry.

References