Difference between revisions of "Part:BBa K3110007"

Revision as of 17:52, 18 October 2019

Medium Promoter Strong RBS lldD

L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1202
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 639

@@ Line 4: / Line 4: @@
 L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a strong RBS.
-lldD cleaves L-lactate to pyruvate. Medium promoter and strong RBS ensures  medium rate of lldD production. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
+lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of
+L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.