Difference between revisions of "Part:BBa K3105676:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | Functionality in Pichia pastoris | + | Functionality in Pichia pastoris <br> |
+ | A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZαB vector are present before the actual terminator starts. | ||
Line 13: | Line 14: | ||
===Source=== | ===Source=== | ||
− | Pichia pastoris | + | Organism : <I>Pichia pastoris</I> <br> |
+ | DNA originates from the pPICZαB vector found in the invitrogen easy select <I>Pichia</I> expression kit | ||
===References=== | ===References=== |
Revision as of 17:36, 18 October 2019
pPICZαB fragment + AOX1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 131
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Functionality in Pichia pastoris
A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZαB vector are present before the actual terminator starts.
Source
Organism : Pichia pastoris
DNA originates from the pPICZαB vector found in the invitrogen easy select Pichia expression kit