Difference between revisions of "Part:BBa K2984009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | <img src="https://2019.igem.org/wiki/images/4/4a/T--Humboldt_Berlin--AR_Paro.jpeg | ||
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| + | <figcaption>Fig.1 - TAP-agar plates containing paromomycin. The <i> C.Reinhardtii </i> colonies growing on the plate were tranformed with our improved <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984006"> paromomycin construct </a> which is flanked by this AR promoter and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018"> Rbcs2 </a> terminator </figcaption> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984009 SequenceAndFeatures</partinfo> | ||
Revision as of 17:35, 18 October 2019
AR Promoter A1-A3
This part is a part of the Chlamy-HUB-Collection. Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 282
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 685
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]