Difference between revisions of "Part:BBa K3228100"
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3228100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3228100 SequenceAndFeatures</partinfo>
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===Description===
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<html>
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<p align="justify">
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This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from <i>Francisella tularensis ssp.</i> novicida, which has been adapted to the MoClo standard.
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</p>
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</html>

Revision as of 17:21, 18 October 2019


pMC_0_4_35_cpf1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1713
    Illegal NheI site found at 3433
    Illegal SpeI site found at 3622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from Francisella tularensis ssp. novicida, which has been adapted to the MoClo standard.