Difference between revisions of "Part:BBa K2984000"
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| − | + | Add more about the biology of this part here | |
===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984000 SequenceAndFeatures</partinfo> | ||
| + | ==Blue & Green Screening== | ||
| + | The effect of the arylsulfatase secretion signal is used for screening of postive Cas9 incision in the SNRK locus in <i>C.reinhardtii</i>. The target site lies within a regultory unit, inhibiting the expression of the arylsulfatse. When cut, expression of the enzyme rises. Outside the cell the acitivity of the enzyme turns a supplemented color into blue proving the functionallity of the signal. | ||
<html> | <html> | ||
Revision as of 17:04, 18 October 2019
3'UTR, Secretion signal of Arylsulfatase ARS B2-B2
This vector is a part of the Chlamy-HUB-Collection. For the secretion of a protein in Chlamydomonas reinhardtii it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.
Add more about the biology of this part here
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Blue & Green Screening
The effect of the arylsulfatase secretion signal is used for screening of postive Cas9 incision in the SNRK locus in C.reinhardtii. The target site lies within a regultory unit, inhibiting the expression of the arylsulfatse. When cut, expression of the enzyme rises. Outside the cell the acitivity of the enzyme turns a supplemented color into blue proving the functionallity of the signal.
