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| | <h1> Improved Version of BBa_K1073024 </h1> | | <h1> Improved Version of BBa_K1073024 </h1> |
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| − | <p>The coat protein with LPETGG (CP-LPETGG) <a href="https://parts.igem.org/Part:BBa_K3187000"target="_blank">(BBa_K3187000)</a>
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| − | consists of 452 amino acids which are encoded by 1359 DNA base pairs. The whole
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| − | protein has a mass of 49.0 kDa. Its relevant parts are the coat protein (CP) <a href="https://parts.igem.org/Part:BBa_K3187017"target="_blank">(BBa_K3187017)</a>
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| − | and the LPETGG sequence <a href="https://parts.igem.org/Part:BBa_K3187019"target="_blank">(BBa_K3187019)</a>.
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| − | <br>LPETGG is a synthetic sequence that is recognized by the enzyme sortase A
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| − | and allows the coupling of CP with other peptides and proteins. For this, the sortase
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| − | cleaves between the amino acids threonine (T) and glycine (G), and threonine forms an amide bond with another
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| − | polyG sequence.
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| − | <sup id="cite_ref-1” class=”reference">
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| − | <a href="#cite_note-1">[1]
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| − | </a>
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| − | </sup>
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| − | We used the Sortase A7M <a href="https://parts.igem.org/Part:BBa_K3187028"target="_blank">(BBa_K3187028)</a>
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| − | and Sortase A5M <a href="https://parts.igem.org/Part:BBa_K3187016"target="_blank">(BBa_K3187016)</a>.
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| − | The polyG recognition sequence is composed of four glycines (GGGG) <a href="https://parts.igem.org/Part:BBa_K3187018"target="_blank">(BBa_K3187018)</a>
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| − | <br>The CP is originally found in bacteriophage P22 and forms its capsid with the scaffold protein(SP)
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| − | <a href="https://parts.igem.org/Part:BBa_K3187021"target="_blank">(BBa_K3187021)</a>.
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| − | Heterologously expressed, CPs and SPs assemble to a Virus-like particle (VLP).
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| − |
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| − | <sup id="cite_ref-2" class="reference">
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| − | <a href="#cite_note-2">[2]
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| − | </a>
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| − | </sup>
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| − | </p>
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| | <!-- Add more about the biology of this part here | | <!-- Add more about the biology of this part here |
