Difference between revisions of "Part:BBa K2984004"
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<figure>
 
<figure>
 
<img src="https://static.igem.org/mediawiki/parts/3/3d/L1c_Transformants.jpeg" alt="colonies_total" width="500">
 
<img src="https://static.igem.org/mediawiki/parts/3/3d/L1c_Transformants.jpeg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - This part was used for our level 1 c backbone. This image shows a successful transformation of the level 1 c backbone with RFP in <i>E. coli</i>, which makes the colonies red. </figcaption>
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<figcaption>Fig.1 - This part was used for our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984002">level 1 c</a> backbone. This image shows a successful transformation of the level 1 c backbone with RFP in <i>E. coli</i>, which makes the colonies red. </figcaption>
 
</figure>
 
</figure>
  

Revision as of 16:49, 18 October 2019


Red fluorescent protein

This part is a part of the Chlamy-HUB-Collection. Red florurescent protein designed for visual screening of E.coli colonies. The red color can be seen with the naked eye, which makes this part especially useful for screening.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 171
    Illegal AgeI site found at 283
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - This part was used for our level 1 c backbone. This image shows a successful transformation of the level 1 c backbone with RFP in E. coli, which makes the colonies red.