Difference between revisions of "Part:BBa K3165005"

 
Line 5: Line 5:
 
sRBS (30000) is a strong ribosome binding site present upstream of the transcriptional unit. sRBS (30000) has a very binding affinity with the ribosomes resulting in enhanced protein expression.
 
sRBS (30000) is a strong ribosome binding site present upstream of the transcriptional unit. sRBS (30000) has a very binding affinity with the ribosomes resulting in enhanced protein expression.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3165005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3165005 SequenceAndFeatures</partinfo>
  
 +
===Usage and Biology===
 +
 +
<h2> Biology </h2>
 +
 +
A Ribosome Binding Site (or RBS) is a short DNA sequence present upstream of the transcriptional unit. In prokaryotic systems, it is easily identified by the presence of a Shine-Dalgarno like sequence which pairs with the anti-Shine-Dalgarno like sequence of the 3' end of 16S rRNA. An RBS is crucial for the process of translation as it dictates the binding of the ribosome (the translational machinery) to the mRNA transcript encoding the protein of interest.
 +
The effectiveness (or strength) of the RBS sequence depends on the base-pairing potential of the RBS to the 16S rRNA along with the spacing between the sequence and the start codon. sRBS (30000) happens to be a strong ribosome binding site enhancing translational efficiency.
 +
 +
<h2> Usage </h2>
 +
 +
The sRBS sequence can be used upstream of any gene whose product is desired to be enhanced. This part is used to maximise the translational efficiency of the CcaR protein in the composite gene constructs.
 +
 +
<h3> References : </h3>
 +
 +
(1) A miniaturized E. coli green light sensor with high dynamic range. Ong NTX, Tabor JJ. Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007. 10.1002/cbic.201800007 PubMed 29420866
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:23, 18 October 2019


sRBS (30000)

sRBS (30000) is a strong ribosome binding site present upstream of the transcriptional unit. sRBS (30000) has a very binding affinity with the ribosomes resulting in enhanced protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

A Ribosome Binding Site (or RBS) is a short DNA sequence present upstream of the transcriptional unit. In prokaryotic systems, it is easily identified by the presence of a Shine-Dalgarno like sequence which pairs with the anti-Shine-Dalgarno like sequence of the 3' end of 16S rRNA. An RBS is crucial for the process of translation as it dictates the binding of the ribosome (the translational machinery) to the mRNA transcript encoding the protein of interest. The effectiveness (or strength) of the RBS sequence depends on the base-pairing potential of the RBS to the 16S rRNA along with the spacing between the sequence and the start codon. sRBS (30000) happens to be a strong ribosome binding site enhancing translational efficiency.

Usage

The sRBS sequence can be used upstream of any gene whose product is desired to be enhanced. This part is used to maximise the translational efficiency of the CcaR protein in the composite gene constructs.

References :

(1) A miniaturized E. coli green light sensor with high dynamic range. Ong NTX, Tabor JJ. Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007. 10.1002/cbic.201800007 PubMed 29420866