Difference between revisions of "Part:BBa K2963004"
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This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme simultaneously coexpressing PgsBCA complex in order to try to make poly-gamma-glutamic acid whose D-glutamate content is different. | This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme simultaneously coexpressing PgsBCA complex in order to try to make poly-gamma-glutamic acid whose D-glutamate content is different. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate. | ||
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| + | ===Characterization=== | ||
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| + | We used enzyme activity data to prove the usability of our racE. We measured the enzymatic activity under different tac promoters. | ||
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| + | [[image:figure2.png|400px]] | ||
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| + | We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used. | ||
| + | <!-- Add more about the biology of this part here | ||
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Revision as of 15:38, 18 October 2019
racE - a glutamate racemase from Bacillus sp.
This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme simultaneously coexpressing PgsBCA complex in order to try to make poly-gamma-glutamic acid whose D-glutamate content is different.
Usage and Biology
The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate.
Characterization
We used enzyme activity data to prove the usability of our racE. We measured the enzymatic activity under different tac promoters.
We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]