Difference between revisions of "Part:BBa K3190101:Design"

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<ul>
 
   <li>Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943</li>
 
   <li>Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943</li>
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   <li>Reference 2</li>
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   <li>G-protein coupled estrogen receptor 1 [Homo sapiens] NCBI Reference Sequence: NP_001091671.1 </li>
 
   <li>Reference 3</li>
 
   <li>Reference 3</li>
 
</ul>
 
</ul>

Revision as of 15:32, 18 October 2019


G protein-coupled estrogen receptor (GPER/GPR30) CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750


Design Notes

The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 (BBa_K3190002) for heterologous expression in S. cerevisiae.


Source

Homo sapiens

References

  • Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. Science, 307(5715), 1625–1630. doi: 10.1126/science.1106943
  • G-protein coupled estrogen receptor 1 [Homo sapiens] NCBI Reference Sequence: NP_001091671.1
  • Reference 3