This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a>Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on
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This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a> They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature. They are either fluorescent reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
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Revision as of 15:23, 18 October 2019
pMC_0_4_22_NanoLuc-K1159001
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 19
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4 Illegal AgeI site found at 520
1000
COMPATIBLE WITH RFC[1000]
Description
specific text here
This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
experimental dataand literature. They are either fluorescent reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
Compability
These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
