Difference between revisions of "Part:BBa K2915300"

 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K2915300 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2915300 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K2915300 parameters</partinfo>
 
<partinfo>BBa_K2915300 parameters</partinfo>
 
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===Design Notes===
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This biobrick is in RFC 10 standards with:
 +
 +
The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
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 +
This NaIII-methylyse exists with:
 +
 +
- with a His-tag and a TEV site

Revision as of 13:28, 18 October 2019


NaIII-methylase-CBD 6-His-Tag with TEV site

NlaIII is a methylase which is part of an enzymes groupes in part of transferase familly, and tranfert a donor methyl groupe into a an acceptor. This enzyme in our project can allows the binding of the "amylase gene" into the cellulose membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This NaIII-methylyse exists with:

- with a His-tag and a TEV site