Difference between revisions of "Part:BBa K2915150:Design"

 
 
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We add a 6Hig-Tag onto the "AFP protein" sequence which allows us to purify our proteins.
 
We add a 6Hig-Tag onto the "AFP protein" sequence which allows us to purify our proteins.
 
We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f APF protein in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.
 
We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f APF protein in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.
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Latest revision as of 13:04, 18 October 2019


IPTG inducible promoter (T7) with RBS AFP-lu1 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a 6Hig-Tag onto the "AFP protein" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f APF protein in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.


Source

Nucleotide sequence

References