Difference between revisions of "Part:BBa K2915150"
Line 3: Line 3:
 
<partinfo>BBa_K2915150 short</partinfo>
 
<partinfo>BBa_K2915150 short</partinfo>
  
−
The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.TheAFP protein can be purify because of the presence of a 6His-Tag in the N-terminal of the protein.
+
The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.The AFP protein was produced and purified because of the presence of a 6His-Tag in the N-terminal of the protein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:03, 18 October 2019


IPTG inducible promoter (T7) with RBS AFP-lu1 6-His-Tag with TEV site

The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.The AFP protein was produced and purified because of the presence of a 6His-Tag in the N-terminal of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL1 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.