Difference between revisions of "Part:BBa K3286044"

Revision as of 12:57, 18 October 2019

Bacteriophage Lambda PL, PRM and PR promoters, operators and 5' UTR's

The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.

Ptashne, M. (2004). A genetic switch: phage lambda revisited (3rd ed.). New York: Cold Spring Harbor Laboratory Press.

Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286044 short</partinfo>
-Red fluorescent protein (RFP) and Green fluorescent protein (GFP) downstream of bacteriophage Lambda promoters, UTR's and operator regions OL and OR.
+The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.
-This construct contains the regulatory regions and promoters of bacteriophage Lambda and has been used to asses a for the potential inhibition of bacteriophage LAmbda by CRISPRi.
+Ptashne, M. (2004). A genetic switch: phage lambda revisited (3rd ed.). New York: Cold Spring Harbor Laboratory Press.
+Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204
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