Difference between revisions of "Part:BBa K2915100:Design"
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Latest revision as of 12:56, 18 October 2019
AFP-Iu1 6-His-Tag with TEV site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add a 6Hig-Tag onto the "antifreeze protein" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain.
Source
nucleics acids