Difference between revisions of "Part:BBa K2915100:Design"

 
(→‎Source)
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
ATGGAGTACTTCTTTCTGCTTATCGTATTGGCAATTCGTACGGTTTCGTCCGATGGGACCTGTGTGAACACCAATTCGCAAATCACCGCCAACTCACAGTGTGTAAAAAGTACTGCTACA AATTGTTACATTGACAACAGCCAACTGGTGGACACATCCATCTGTACCCGCAGTCAGTATTCGGACGCCAACGTGAAAAAGAGCGTCACCACTGATTGCAATATTGACAAGTCCCAGGTA TATCTTACTACCTGTACTGGTAGTCAGTACAATGGCATCTACATCCGTTCATCGACGACTACCGGGACGAGCATTAGTGGACCCGGTTGTAGTATTTCGACCTGCACCATTACGCGTGGT GTAGCCACACCCGCGGCAGCTTGTAAAATTTCAGGTTGTTCTTTGTCGGCAATG
+
nucleic acid
  
 
===References===
 
===References===

Revision as of 12:56, 18 October 2019


AFP-Iu1 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a 6Hig-Tag onto the "antifreeze protein" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain.


Source

nucleic acid

References