Difference between revisions of "Part:BBa K2915100"

 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K2915100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2915100 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K2915100 parameters</partinfo>
 
<partinfo>BBa_K2915100 parameters</partinfo>
 
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===Design Notes===
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This biobrick is in RFC 10 standards with:
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 +
The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
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This AFP-lu1 exists with:
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- with a His-tag and a TEV site

Revision as of 12:52, 18 October 2019


AFP-Iu1 6-His-Tag with TEV site

The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This AFP-lu1 exists with:

- with a His-tag and a TEV site