Difference between revisions of "Part:BBa K2915225"
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<partinfo>BBa_K2915225 short</partinfo>
 
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The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria. We also tested production of the protein and used the his-tag to purify the protein.  
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The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CTGATC3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha/BL21 E3 bacteria. We also tested production of the protein and used the His-Tag to purify the protein.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K2915225 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2915225 SequenceAndFeatures</partinfo>
  

Revision as of 12:46, 18 October 2019


IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site

The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CTGATC3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha/BL21 E3 bacteria. We also tested production of the protein and used the His-Tag to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL1 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.