Difference between revisions of "Part:BBa K3160021"
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The expression of miR-196a and miR-148a were found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a miR-148a sensor contain two complementary binding sites to miR-196a miR-148a which inhibit miR-196a and miR-148aexpression. The sequence of miR-196a and miR-148asensor wrere synthetised by GenScript (Shanghai, China). We inserted miR-196a miR-148a sensor into the 3' end of GFP, which generated GFP- miR-196a miR-148a sensor.
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The expression of miR-21 and miR-17 were found to be significantly associated with all tumor stage of gastric cancer. We designed miR-21-17 sensor contain complementary binding sites to miR-21 and miR-17 which inhibit miR-21 and miR-17 expression. The sequences of miR-21-17 sensor were synthesized by GenScript (Shanghai, China). We inserted miR-21-17 sensor into the 3’UTR of mcherry, which generated mcherry -miR-21-17 sensor.

Revision as of 12:29, 18 October 2019


MCherry-miR-21-17 sensor

The expression of miR-21 and miR-17 were found to be significantly associated with all tumor stage of gastric cancer. We designed miR-21-17 sensor contain complementary binding sites to miR-21 and miR-17 which inhibit miR-21 and miR-17 expression. The sequences of miR-21-17 sensor were synthesized by GenScript (Shanghai, China). We inserted miR-21-17 sensor into the 3’UTR of mcherry, which generated mcherry -miR-21-17 sensor.