Difference between revisions of "Part:BBa K3102046"

Revision as of 12:25, 18 October 2019

Electrons production (pflB, ACS, FDH, MDH)

This “Electrons production” composite part is composed of pyruvate formate-lyase (pflB: BBa_K3102037), acetyl-CoA synthetase (ACS: BBa_K3102038), NAD-dependent formate dehydrogenase (FDH: BBa_K3102039) as well as the malate dehydrogenase (MDH: BBa_K3102040).

The pflB oxidizes pyruvate and acetyl-CoA while ACS induces the transformation of acetate into acetyl-CoA. Additionally, FDH and MDH lead to generation of cofactors and the oxidation of malate into oxaloacetate. Those are responsible for the production of reduced coenzymes generating electrons and all genes are overexpressed to enhance these mechanisms.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 6180
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 5516
Illegal AgeI site found at 534
Illegal AgeI site found at 3413
Illegal AgeI site found at 6024
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 836

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3102046 short</partinfo>
-a entrer
+This “Electrons production” composite part is composed of pyruvate formate-lyase (pflB: BBa_K3102037), acetyl-CoA synthetase (ACS: BBa_K3102038),  NAD-dependent formate dehydrogenase (FDH: BBa_K3102039) as well as the malate dehydrogenase (MDH: BBa_K3102040).
+The pflB oxidizes pyruvate and acetyl-CoA while ACS induces the transformation of acetate into acetyl-CoA. Additionally, FDH and MDH lead to generation of cofactors and the oxidation of malate into oxaloacetate. Those are responsible for the production of reduced coenzymes generating electrons and all genes are overexpressed to enhance these mechanisms.
 <!-- Add more about the biology of this part here