Difference between revisions of "Part:BBa K2915225"
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<partinfo>BBa_K2915225 short</partinfo> | <partinfo>BBa_K2915225 short</partinfo> | ||
− | The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. | + | The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.The promoter T7 and RBS (Bba_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria. |
Revision as of 12:24, 18 October 2019
IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site
The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.The promoter T7 and RBS (Bba_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick is in RFC 10 standards with:
The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
This TAL1 exists with:
- with a His-tag and a TEV site
- with a promotor T7 and RBS (BBa_K525998) which is the regulator.