Difference between revisions of "Part:BBa K2921620"

 
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This construct constitutively expresses a colored metal-binding fusion protein: OprF linked with mRFP.
 
This construct constitutively expresses a colored metal-binding fusion protein: OprF linked with mRFP.
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https://2019.igem.org/wiki/images/7/72/T--TAS_Taipei--lqK2921620.jpg
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:43, 18 October 2019


Promoter + RBS + OprF + mRFP + Double Terminator

This construct constitutively expresses a colored metal-binding fusion protein: OprF linked with mRFP.

T--TAS_Taipei--lqK2921620.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 663
    Illegal AgeI site found at 1251
    Illegal AgeI site found at 1363
  • 1000
    COMPATIBLE WITH RFC[1000]