Difference between revisions of "Part:BBa K2921150"
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This construct constitutively expresses Leaf Compost Cutinase (Basic part: BBa_K936000) linked with amilCP (Basic part: BBa_K592009). Cutinases are lypolytic/esterolytic enzymes that degrade and hydrolyze the ester bonds of cutin. The amilCP serves as a functional color reporter, allowing for convenient visible detection of the location of LC Cutinase. | This construct constitutively expresses Leaf Compost Cutinase (Basic part: BBa_K936000) linked with amilCP (Basic part: BBa_K592009). Cutinases are lypolytic/esterolytic enzymes that degrade and hydrolyze the ester bonds of cutin. The amilCP serves as a functional color reporter, allowing for convenient visible detection of the location of LC Cutinase. | ||
https://2019.igem.org/wiki/images/b/be/T--TAS_Taipei--lqK2921150.jpg | https://2019.igem.org/wiki/images/b/be/T--TAS_Taipei--lqK2921150.jpg | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:06, 18 October 2019
6xHIS+LC Cutinase+GS+amilCP
This construct constitutively expresses Leaf Compost Cutinase (Basic part: BBa_K936000) linked with amilCP (Basic part: BBa_K592009). Cutinases are lypolytic/esterolytic enzymes that degrade and hydrolyze the ester bonds of cutin. The amilCP serves as a functional color reporter, allowing for convenient visible detection of the location of LC Cutinase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 716
- 1000COMPATIBLE WITH RFC[1000]