Difference between revisions of "Part:BBa K3204002"

Revision as of 09:30, 18 October 2019

Receptor binding protein of paenibacillus phage HB10c2

This is the putative Receptor binding protein of the paenibacillus phage HB10c2 with the DNA sequence codon-optimized for the expression in E. coli. It binds an unknown receptor on Paenibacillus larvae, the pathogen causing American foulbrood. The binding is very specific to Paenibacillus larvae.

The protein has been identified in silico as the most promising. Modeling of several genes in the cluster of structural proteins of HB10c2 with the webtool Phyre2 has led us to propose the gene order (...) - tail length tape measure protein (14) - dit (15) - tal (16) - RBP (17) - (unknown proteins) - lysin (21) - (...). This order resembles well known clusters of baseplate coding regions for other Siphoviridae phages.

The binding has not been proven with sufficiently reliable experimental data. The experiment suggest, that the binding could have worked, but the data is not resilient enough to claim it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 649
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3204002 short</partinfo>
-This is the putative Receptor binding protein of the paenibacillus phage HB10c2 with the Codon sequence optimized for the expression in <i>E. coli</i>. It binds an unknown receptor on <i>Paenibacillus larvae</i>, the pathogen causing American foulbrood. The binding is very specific to <i>Paenibacillus larvae</i>.
+This is the putative Receptor binding protein of the paenibacillus phage HB10c2 with the DNA sequence codon-optimized for the expression in <i>E. coli</i>. It binds an unknown receptor on <i>Paenibacillus larvae</i>, the pathogen causing American foulbrood. The binding is very specific to <i>Paenibacillus larvae</i>.
-The binding has not been proven with sufficiently reliable data. The experiments suggest, that the binding could have worked, but the data is not resilient.
+The protein has been identified in silico as the most promising. Modeling of several genes in the cluster of structural proteins of HB10c2 with the webtool Phyre2 has led us to propose the gene order (...) - tail length tape measure protein (14) - dit (15) - tal (16) - RBP (17) - (unknown proteins) - lysin (21) - (...). This order resembles well known clusters of baseplate coding regions for other Siphoviridae phages.
+The binding has not been proven with sufficiently reliable experimental data. The experiment suggest, that the binding could have worked, but the data is not resilient enough to claim it.
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