Difference between revisions of "Part:BBa K1614009"
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===Usage and Biology===
 
===Usage and Biology===
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1.Our vision & The parts we characterize
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Team DUT_China_A 2019 dedicate themselves to convey the concept of functional DNA application across iGEM and we would like to provide iGEM conmunity with available functional DNA. Therefore, we add experimental characterization data to the aptamers constructed by Team Heidelberg and we improve their parts.
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They conceived and implemented an open-source software for the design of aptamers (MAWS) as a fast and affordable alternative to the laborious SELEX procedure.
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1.BBa_K1614009- software generated kanamycin aptamer (candidate 1)
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2.BBa_K1614013- software generated kanamycin aptamer (candidate 2)
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3.BBa_K1614017- software generated kanamycin aptamer (candidate 3)
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To test these aptamers whether they can bind to kanamycin, we put forward a plan, Gold nanoparticle-based colorimetric detection.
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Revision as of 08:01, 18 October 2019

software generated kanamycin aptamer (candidate 1)

Notice: Functional DNA

This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.

Aptamer binding kanamycin created by the MAWS (http://2015.igem.org/Team:Heidelberg/software/maws) Software from iGEM Team Heidelberg, further validated by an HRP based assay (http://2015.igem.org/Team:Heidelberg/project/hlpd).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]