Difference between revisions of "Part:BBa K3239002:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
the 428th nucleotide (A) was changed to (T) to be TypeIIS compatible.
+
the 428th nucleotide (A) was changed to (T) for Type IIS compatibility.
 
+
 
+
  
 
===Source===
 
===Source===

Revision as of 05:44, 18 October 2019


Promoter for the Mit1 transcription factor in the P.pastoris GS115 strain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the 428th nucleotide (A) was changed to (T) for Type IIS compatibility.

Source

P. pastoris GS115 strain.

References