Difference between revisions of "Part:BBa K3254027"

Revision as of 05:38, 18 October 2019

Translational Unit for Int7 integrase

This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int7 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 389
Illegal PstI site found at 938
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 80
Illegal PstI site found at 389
Illegal PstI site found at 938
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 389
Illegal PstI site found at 938
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 389
Illegal PstI site found at 938
Illegal AgeI site found at 929
Illegal AgeI site found at 1330
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3254027 short</partinfo>
-This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int5 attP and attB will happen as expected. The RiboJ([[Part:BBa_K2615996|BBa_K2615996]]) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.
+This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int7 attP and attB will happen as expected. The RiboJ([[Part:BBa_K2615996|BBa_K2615996]]) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.
 <!-- Add more about the biology of this part here