Difference between revisions of "Part:BBa K2989001"
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<partinfo>BBa_K2989001 short</partinfo> | <partinfo>BBa_K2989001 short</partinfo> | ||
| − | + | SCP1 is a small phosphatase of the C-terminal domain polypeptide of RNA polymerase II, The sequence of the 31 to 55 position of SCP1 has a membrane localization effect in various cell lines, such as human embryonic kidney cell HEK293T, human colon cancer cell DLD1, and human breast cancer epithelial cell MCF-7. Hepatitis B surface antigen is the main immunogen of host protective immunity, which can stimulate the body to produce neutralizing antibodies. In its S region protein, a fragment of 92 amino acids in length has strong immunogenicity. | |
| − | + | ||
| − | + | ===Usage=== | |
| − | === | + | We first placed the SCP1 membrane localization sequence at the N-terminus of the selected "Trojan horse" fragment (92 amino acid length HBsAg fragment) to exert its membrane localization function, prompting the "Trojan horse" fragment to be expressed on the cell membrane. . Because the "Trojan horse" fragment has strong CD4+T and CD8+ T cell epitopes, it can trigger the body's own immune response to produce a killing effect on tumor cells expressing the "Trojan horse" fragment. |
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| + | ===Biology=== | ||
| + | The serine/threonine phosphatase (PSP) in the protein contains three major families: phosphorylated protein phosphoprotein esterases (PPPs), metal-dependent phosphatases (PPMs), and aspartate-based phosphatases. Among them, SCP1 belongs to the third family, an aspartate-based phosphatase. It is highly conserved from fruit flies to mammals. The phosphorylated target protein can be regulated by dephosphorylation by recognizing a conserved SP sequence on the substrate. SCP1 is able to inhibit the expression of neural genes in non-neuronal cells; it can regulate the activity of RNA polymerase and inhibit transcription. It also stabilizes the expression of Snail protein, which promotes cell migration in breast cancer. | ||
| + | Hepatitis B surface antigen (HBsAg) is an outer membrane protein of HBV encoded by the HBV S gene region. The S gene is divided into a preS1 region, a preS2 region, and an S region, which has respective start codons (ATGs) but shares a stop codon (TGA) encoding PreS1 protein, PreS2 protein, and S protein, respectively. The S protein is a small protein (SP), also known as the major protein, consisting of 226 amino acids. The 146th asparagine (Asn) residue is a glycosylation site, a non-glycosylated form. The molecular weight is 24 kDa, and the glycosylated form has a molecular weight of about 27 kDa, of which the main protein is the main component of HBsAg. Hepatitis B surface antigen is the main immunogen of host protective immunity, which can stimulate the body to produce neutralizing antibodies, also known as protective antibodies HBsAb. HBs Ab has a strong neutralizing effect on HBV. The general appearance of HBsAb marks the termination of HBV infection and the body has specific immunity to HBV. | ||
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Revision as of 03:25, 18 October 2019
SCP1 -HBsAg
SCP1 is a small phosphatase of the C-terminal domain polypeptide of RNA polymerase II, The sequence of the 31 to 55 position of SCP1 has a membrane localization effect in various cell lines, such as human embryonic kidney cell HEK293T, human colon cancer cell DLD1, and human breast cancer epithelial cell MCF-7. Hepatitis B surface antigen is the main immunogen of host protective immunity, which can stimulate the body to produce neutralizing antibodies. In its S region protein, a fragment of 92 amino acids in length has strong immunogenicity.
Usage
We first placed the SCP1 membrane localization sequence at the N-terminus of the selected "Trojan horse" fragment (92 amino acid length HBsAg fragment) to exert its membrane localization function, prompting the "Trojan horse" fragment to be expressed on the cell membrane. . Because the "Trojan horse" fragment has strong CD4+T and CD8+ T cell epitopes, it can trigger the body's own immune response to produce a killing effect on tumor cells expressing the "Trojan horse" fragment.
Biology
The serine/threonine phosphatase (PSP) in the protein contains three major families: phosphorylated protein phosphoprotein esterases (PPPs), metal-dependent phosphatases (PPMs), and aspartate-based phosphatases. Among them, SCP1 belongs to the third family, an aspartate-based phosphatase. It is highly conserved from fruit flies to mammals. The phosphorylated target protein can be regulated by dephosphorylation by recognizing a conserved SP sequence on the substrate. SCP1 is able to inhibit the expression of neural genes in non-neuronal cells; it can regulate the activity of RNA polymerase and inhibit transcription. It also stabilizes the expression of Snail protein, which promotes cell migration in breast cancer. Hepatitis B surface antigen (HBsAg) is an outer membrane protein of HBV encoded by the HBV S gene region. The S gene is divided into a preS1 region, a preS2 region, and an S region, which has respective start codons (ATGs) but shares a stop codon (TGA) encoding PreS1 protein, PreS2 protein, and S protein, respectively. The S protein is a small protein (SP), also known as the major protein, consisting of 226 amino acids. The 146th asparagine (Asn) residue is a glycosylation site, a non-glycosylated form. The molecular weight is 24 kDa, and the glycosylated form has a molecular weight of about 27 kDa, of which the main protein is the main component of HBsAg. Hepatitis B surface antigen is the main immunogen of host protective immunity, which can stimulate the body to produce neutralizing antibodies, also known as protective antibodies HBsAb. HBs Ab has a strong neutralizing effect on HBV. The general appearance of HBsAb marks the termination of HBV infection and the body has specific immunity to HBV.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]