Difference between revisions of "Part:BBa K2926057"
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| − | <span class='h3bb'><h1>Sequence and Features<h1></span> | + | <span class='h3bb'><h1>Sequence and Features</h1></span> |
Sequence was validated by Sanger sequencing. | Sequence was validated by Sanger sequencing. | ||
<partinfo>BBa_K2926057 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2926057 SequenceAndFeatures</partinfo> | ||
Revision as of 22:55, 17 October 2019
Fusion protein of mating factor alpha, mCherry and pVIII with his-tag
Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]