Difference between revisions of "Part:BBa K2926056"

 
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<partinfo>BBa_K2926056 short</partinfo>
 
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The extracellular domain of S. cerevisiaes membrane protein Opy2 was n-terminally fused to mCherry to enable protein visualization. Additionally, the major coat protein pVIII of the bacteriophage M13 was fused to the c-terminus of mCherry.
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The extracellular domain of <i>S. cerevisiaes</i> membrane protein Opy2 was N-terminally fused to mCherry to enable protein visualization. Additionally, the major coat protein pVIII of the bacteriophage M13 was fused to the C-terminus of mCherry.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h1>Sequence and Features</h1></span>
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Sequence was validated by Sanger sequencing.
 
<partinfo>BBa_K2926056 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2926056 SequenceAndFeatures</partinfo>
  

Revision as of 22:41, 17 October 2019


Fusion protein of Opy2 from yeast, mCherry and pVIII from the bacteriophage M13

The extracellular domain of S. cerevisiaes membrane protein Opy2 was N-terminally fused to mCherry to enable protein visualization. Additionally, the major coat protein pVIII of the bacteriophage M13 was fused to the C-terminus of mCherry.

Sequence and Features

Sequence was validated by Sanger sequencing.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]