Revision as of 21:49, 17 October 2019

Fusion protein of Flo11 (yeast), mCherry and pVIII (M13 bacteriophage) with purification tag To visualize the interactions of the n-terminal lectin binding domain of the S. cerevisiae protein Flo11 we fused the coding sequence n-terminally to the coding sequence of the fluorescence reporter protein mCherry. mCherry was n-terminally fused to the M13 bacteriophage major coat protein pVIII. The whole protein was fused to a C-terminal hexahistidine tag to enable protein purification via metal ions.

Sequence was validated by Sanger sequencing.