Difference between revisions of "Part:BBa K2908671:Design"
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| + | - We use the pLN431 as the back-clone of the Module1, then we cut the vector and an insert sequence (lacZp+lacZ) with MluI and NotI to form the pocket plasmid. | ||
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- We designed the BsmBI restriction digest location on the 5’ and 3’ top of the inserted sequence, the following figure shows the pattern of construction method.<br/> | - We designed the BsmBI restriction digest location on the 5’ and 3’ top of the inserted sequence, the following figure shows the pattern of construction method.<br/> | ||
https://static.igem.org/mediawiki/parts/2/2e/T--CSU_CHINA--ggmodule1.png | https://static.igem.org/mediawiki/parts/2/2e/T--CSU_CHINA--ggmodule1.png | ||
Revision as of 21:35, 17 October 2019
- We use the pLN431 as the back-clone of the Module1, then we cut the vector and an insert sequence (lacZp+lacZ) with MluI and NotI to form the pocket plasmid.
- We designed the BsmBI restriction digest location on the 5’ and 3’ top of the inserted sequence, the following figure shows the pattern of construction method.
