Difference between revisions of "Part:BBa K2908668"
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miRNA-BDs were constructed bearing four repeats of miR-21-5p in the 3’ UTR, or 5’ UTR. Since these miRNA-BDs do not have ATGs, several ATGs were added between the miRNA target sites in some sensors. Extra bases were added to separate the ATGs by a number of bases divisible by three. The distance between the last ATG and the true reporter start codon was either divisible by three or not . | miRNA-BDs were constructed bearing four repeats of miR-21-5p in the 3’ UTR, or 5’ UTR. Since these miRNA-BDs do not have ATGs, several ATGs were added between the miRNA target sites in some sensors. Extra bases were added to separate the ATGs by a number of bases divisible by three. The distance between the last ATG and the true reporter start codon was either divisible by three or not . | ||
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| − | + | <span class='h3bb'>Sequence and Features</span> | |
<partinfo>BBa_K2908668 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2908668 SequenceAndFeatures</partinfo> | ||
Revision as of 21:15, 17 October 2019
miR101-BD
When this part binding with miR101, it can inhibit the translation of mRNA which include the same sequence.This part is designed by assembling several complementary sequence with miR101 together and synthesized by company.It is used as a target aimed by miR101 to block the gene circuit in normal cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]