Difference between revisions of "Part:BBa K2908667"
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<partinfo>BBa_K2908667 short</partinfo><br/> | <partinfo>BBa_K2908667 short</partinfo><br/> | ||
https://static.igem.org/mediawiki/parts/6/64/T--CSU_CHINA--yCD.png<br/> | https://static.igem.org/mediawiki/parts/6/64/T--CSU_CHINA--yCD.png<br/> | ||
| + | This figure shows the crystal structure of yeast cytosine deaminase (yCD).<br/> | ||
The cytosine deaminase (CD)/5-fluorocytosine (5-FC) approach is the next most widely studied suicide gene therapy approach. CD is uniquely expressed in certain fungi and bacteria and it converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death. | The cytosine deaminase (CD)/5-fluorocytosine (5-FC) approach is the next most widely studied suicide gene therapy approach. CD is uniquely expressed in certain fungi and bacteria and it converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
https://static.igem.org/mediawiki/parts/f/f2/T--CSU_CHINA--yCD1.png<br/> | https://static.igem.org/mediawiki/parts/f/f2/T--CSU_CHINA--yCD1.png<br/> | ||
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Revision as of 21:11, 17 October 2019
yCD
This figure shows the crystal structure of yeast cytosine deaminase (yCD).
The cytosine deaminase (CD)/5-fluorocytosine (5-FC) approach is the next most widely studied suicide gene therapy approach. CD is uniquely expressed in certain fungi and bacteria and it converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death.
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 854
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 466
Illegal BsaI.rc site found at 503