Difference between revisions of "Part:BBa K2908000"
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https://static.igem.org/mediawiki/2019/7/77/T--CSU_CHINA--s(GATA3)p.png | https://static.igem.org/mediawiki/2019/7/77/T--CSU_CHINA--s(GATA3)p.png | ||
- Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity. | - Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity. | ||
| − | https://static.igem.org/mediawiki/parts/6/66/T--CSU_CHINA--JASPER-GATA3.png | + | < img src="https://static.igem.org/mediawiki/parts/6/66/T--CSU_CHINA--JASPER-GATA3.png" align="center" /> |
This figure shows the results we browse using JASPAR by input homo sapiens GATA3. | This figure shows the results we browse using JASPAR by input homo sapiens GATA3. | ||
Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter. | Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter. | ||
Revision as of 20:16, 17 October 2019
p.png)
- Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity.
< img src="
" align="center" />
This figure shows the results we browse using JASPAR by input homo sapiens GATA3.
Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter.