Difference between revisions of "Part:BBa K2908000"
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| − | https://static.igem.org/mediawiki/2019/ | + | https://static.igem.org/mediawiki/2019/7/77/T--CSU_CHINA--s(GATA3)p.png |
- Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity. | - Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity. | ||
| − | https://static.igem.org/mediawiki/2019/ | + | https://static.igem.org/mediawiki/2019/7/77/T--CSU_CHINA--JASPER-GATA3.png |
This figure shows the results we browse using JASPAR by input homo sapiens GATA3. | This figure shows the results we browse using JASPAR by input homo sapiens GATA3. | ||
Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter. | Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter. | ||
Revision as of 20:12, 17 October 2019
p.png)
- Construction: We use the JASPER to get the TF binding sites (TF-BSs), and add different spacers between the tandem repeats of TF-BSs to avoid creating new binding sites in order to ensure the specificity.
This figure shows the results we browse using JASPAR by input homo sapiens GATA3.
Then we add a strong and short promoter named lateADEp to ensure the strength of the whole promoter.