Difference between revisions of "Part:BBa K2993000"
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This part is special in its own way because it actually is a fusion of one half of a protein to a whole array of zinc-finger proteins. Much like a multi fusion protein. | This part is special in its own way because it actually is a fusion of one half of a protein to a whole array of zinc-finger proteins. Much like a multi fusion protein. | ||
| + | |||
| + | The full sequence of this composite is as follows: | ||
| + | GAATTCGCGGCCGCTTCTAGAGCCTGCAATGACGGACGTATTCCTCTGGCCTCAACGCAAGATTGGCAGCTGAAACCGCCTTTCCAAAGTGA | ||
| + | GTCCTTCGTCTATACGTTGCTTCTCAACGTGGCAAGTGACAGCACCACTGTGAACCAAACGTCCCGTTTCCACGTACCCAAGGGTGCCCAAT | ||
| + | CTTGTATTAAGCattaaagaggagaaaaCTAGATGGCCGGCCACCACCATCACCATCACCATCACACCGGCggagaaagcttgtttaagggg | ||
| + | ccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttgg | ||
| + | tcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaaca | ||
| + | ccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaa | ||
| + | tttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactACCGGCGGAGGGTCGGCAGGAGGGAGTGGTTCCGG | ||
| + | ATCTAGTGGTGGAAGCTCAGGAGCTAGTGGTACAGGCACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGCCTGGAGC | ||
| + | CGGGTGAAAAACCGTACAAATGCCCTGAATGCGGTAAAAGCTTTAGTCGCAGCGACAAACTTGTGCGCCACCAGCGTACTCACACCGGCGAA | ||
| + | AAACCATATAAATGCCCGGAGTGTGGCAAAAGCTTCAGTCGCGAAGATAACCTTCACACCCACCAGCGCACCCATACCGGCGAAAAGCCGTA | ||
| + | TAAATGCCCAGAATGTGGCAAAAGCTTTAGCCATCGCACGACCCTGACCAACCACCAGCGCACCCACACCGGCGAGAAACCGTATAAATGTC | ||
| + | CCGAGTGTGGAAAGTCTTTCTCTACAAGTGGCCATTTGGTGCGTCATCAACGTACTCACACCGGAGAAAAGCCGTACAAATGCCCAGAATGC | ||
| + | GGTAAGTCCTTCTCTCGTGAGGACAACTTGCATACCCATCAACGCACGCACACGGGTGAGAAACCATATAAGTGCCCCGAGTGCGGAAAATC | ||
| + | GTTTTCTCAGTCAAGTTCCTTAGTCCGCCATCAGCGCACACACACTGGGAAGAAGACTAGCTACTAGTAGCGGCCGCTGCAG | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 20:09, 17 October 2019
Fusion protein of N-TEV-GSAT-ZincFingerArray1
This part contains the N-TEV split protein fused to a zincfinger array via a GSAT-linker.
This fusion protein should be used with its counterpart which contains the C-TEV protein fused to another zinc-finger array with a binding affinity for a different DNA sequence. On our iGEMteam_Rotterdam2019 webpage, you can read all about how to use this part properly.
This part is special in its own way because it actually is a fusion of one half of a protein to a whole array of zinc-finger proteins. Much like a multi fusion protein.
The full sequence of this composite is as follows: GAATTCGCGGCCGCTTCTAGAGCCTGCAATGACGGACGTATTCCTCTGGCCTCAACGCAAGATTGGCAGCTGAAACCGCCTTTCCAAAGTGA GTCCTTCGTCTATACGTTGCTTCTCAACGTGGCAAGTGACAGCACCACTGTGAACCAAACGTCCCGTTTCCACGTACCCAAGGGTGCCCAAT CTTGTATTAAGCattaaagaggagaaaaCTAGATGGCCGGCCACCACCATCACCATCACCATCACACCGGCggagaaagcttgtttaagggg ccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttgg tcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaaca ccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaa tttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactACCGGCGGAGGGTCGGCAGGAGGGAGTGGTTCCGG ATCTAGTGGTGGAAGCTCAGGAGCTAGTGGTACAGGCACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGCCTGGAGC CGGGTGAAAAACCGTACAAATGCCCTGAATGCGGTAAAAGCTTTAGTCGCAGCGACAAACTTGTGCGCCACCAGCGTACTCACACCGGCGAA AAACCATATAAATGCCCGGAGTGTGGCAAAAGCTTCAGTCGCGAAGATAACCTTCACACCCACCAGCGCACCCATACCGGCGAAAAGCCGTA TAAATGCCCAGAATGTGGCAAAAGCTTTAGCCATCGCACGACCCTGACCAACCACCAGCGCACCCACACCGGCGAGAAACCGTATAAATGTC CCGAGTGTGGAAAGTCTTTCTCTACAAGTGGCCATTTGGTGCGTCATCAACGTACTCACACCGGAGAAAAGCCGTACAAATGCCCAGAATGC GGTAAGTCCTTCTCTCGTGAGGACAACTTGCATACCCATCAACGCACGCACACGGGTGAGAAACCATATAAGTGCCCCGAGTGCGGAAAATC GTTTTCTCAGTCAAGTTCCTTAGTCCGCCATCAGCGCACACACACTGGGAAGAAGACTAGCTACTAGTAGCGGCCGCTGCAG
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 339