Difference between revisions of "Part:BBa K145151:Design"

 
(Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K145151 short</partinfo>
 
<partinfo>BBa_K145151 short</partinfo>
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===Design Notes===
 
===Design Notes===
just one stop codon in the end
+
This part was made with a PCR of template [https://parts.igem.org/Part:BBa_P1010 BBa_P1010]. The primers used were:
 
+
primer 1: CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC
 
+
primer 2: CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG
  
 
===Source===
 
===Source===

Latest revision as of 12:47, 14 October 2008

ccdB coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

This part was made with a PCR of template BBa_P1010. The primers used were:

primer 1: CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC
primer 2: CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG

Source

P1010

References