Difference between revisions of "Part:BBa K3013000"

m
m
Line 1: Line 1:
  
−
<!-- This will include a dynamicaly generated element by the page -->
+
__NOTOC__
−
</html><partinfo>BBa_K3013000 short</partinfo><html>
+
<partinfo>BBa_K3013000 short</partinfo>
  
 
LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
 
LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
Line 7: Line 7:
 
We used this part to tag polypropylene and polystyrene with eGFP.  
 
We used this part to tag polypropylene and polystyrene with eGFP.  
  
−
 
+
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
−
Therefore we made a fusion protein: eGFP - 17x helix - LCI.
+
<!-- -->
−
This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates.
+
−
 
+
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
 
+
<partinfo>BBa_K3013001 SequenceAndFeatures</partinfo>
−
<!-- This will include a dynamicaly generated element by the page -->
+
−
</html><partinfo>BBa_K3013000 SequenceAndFeatures</partinfo><html>
+
  
  
 +
<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
−
</html><partinfo>BBa_K3013000 parameters</partinfo><html>
+
<partinfo>BBa_K3013001 parameters</partinfo>
−
 
+
<!-- -->
−
<p>
+
−
Example Text<br>
+
−
<br>
+
−
Everything in here works like in a normal website.<br>
+
−
So a newline is created with &lt;br&gt;<br>
+
−
</p>
+
−
<img title="nice title" src="//2019.igem.org/wiki/images/0/0e/T--aachen--favicon.png"/>
+
−
<p>
+
−
Example Text<br>
+
−
<br>
+
−
Everything in here works like in a normal website.<br>
+
−
So a newline is created with &lt;br&gt;<br>
+
−
</p>
+
−
 
+
−
</html>
+

Revision as of 17:08, 17 October 2019


LCI, polymer binding peptide

LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.

We used this part to tag polypropylene and polystyrene with eGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]