Difference between revisions of "Part:BBa K3282005"

 
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<partinfo>BBa_K3282005 short</partinfo>
 
<partinfo>BBa_K3282005 short</partinfo>
  
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The part consists of T7 promoter (BBa_I719005), two Anderson RBS (BBa_J61109) separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (BBa_B0012).
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The part consists of T7 promoter [https://parts.igem.org/Part:BBa_I719005 (BBa_I719005)], two Anderson RBS [https://parts.igem.org/Part:BBa_J61109 (Part:BBa_J61109)], separating two coding regions of lead binding protein [https://parts.igem.org/Part:BBa_K3282002 (BBa_K3282002)] and lead transport protein [https://parts.igem.org/Part:BBa_K3282001 (BBa_K3282001)], followed by a TE terminator [https://parts.igem.org/Part:BBa_B0012 (Part:BBa_B0012)].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:54, 17 October 2019


pbrD and pbrT under T7 promoter


The part consists of T7 promoter (BBa_I719005), two Anderson RBS (Part:BBa_J61109), separating two coding regions of lead binding protein (BBa_K3282002) and lead transport protein (BBa_K3282001), followed by a TE terminator (Part:BBa_B0012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1444
    Illegal NheI site found at 1642
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1392
    Illegal NgoMIV site found at 1855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1999
    Illegal SapI.rc site found at 2229