Difference between revisions of "Part:BBa K145014:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
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<partinfo>BBa_K145014 short</partinfo>
 
<partinfo>BBa_K145014 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
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This part is made using a two-step PCR. The template was part [https://parts.igem.org/Part:BBa_I712022 BBa_I712022] and the primers were:
  
 +
Step I:
 +
primer 1: CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
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primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
  
 +
Step II:
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primer 1: ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
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primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
  
 
===Source===
 
===Source===

Latest revision as of 12:15, 14 October 2008

T7 RNA Polymerase with UmuD derived tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is made using a two-step PCR. The template was part BBa_I712022 and the primers were:

Step I: 

primer 1: CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG

Step II: 

primer 1: ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG

Source

Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)

References