Difference between revisions of "Part:BBa K3226019"

Revision as of 15:22, 17 October 2019

RecA+his

This coding sequence has 6×histidine attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA.

Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.

<Usage>

This is mainly used to obtain resistance to radiation. In our project, we used γ-rays as one type of radiation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 242
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 916
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <Long description>
- This coding sequence has 6×histidine attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA.
+This coding sequence has 6×histidine attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA.
- Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.
+Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.
 <Usage>