Difference between revisions of "Part:BBa K145001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | T7 polymerase from BBa_I712022 with NLS site removed and TAATAA double stop signal added | + | T7 polymerase from BBa_I712022 with NLS site removed and TAATAA double stop signal added. This was done with PCR using the following primers: |
| + | primer 1: ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC | ||
| + | primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG | ||
===Source=== | ===Source=== | ||
Latest revision as of 11:45, 14 October 2008
T7 RNA polymerase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 polymerase from BBa_I712022 with NLS site removed and TAATAA double stop signal added. This was done with PCR using the following primers:
primer 1: ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC primer 2: CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
Source
T7 polymerase from BBa_I712022.