Difference between revisions of "Part:BBa K2924034"
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<partinfo>BBa_K2924034 short</partinfo>
 
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Strong constitutive cyanobacterial promoter [https://parts.igem.org/part:BBa_K2924000 Pcpc560] expressing [https://parts.igem.org/part:BBa_K2924026 a-s1-casein] with the [https://parts.igem.org/part:BBa_B0015 T1/T7 double terminator].
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Strong constitutive cyanobacterial promoter <a href="https://parts.igem.org/part:BBa_K2924000">P<sub>cpc560</sub></a> expressing <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> with the <a href="https://parts.igem.org/part:BBa_B0015">T1/T7 double terminator</a>.
  
 
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The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>.  
 
The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>.  
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After testing the strength of the promoter with a fluorescent reporter [https://parts.igem.org/part:BBa_K2924036 mVenus], it was used to express a protein from cow’s milk, [https://parts.igem.org/part:BBa_K2924026 a-s1-casein] in <i>Synechocystis</i> sp. PCC 6803.
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After testing the strength of the promoter with a fluorescent reporter <a href="https://parts.igem.org/part:BBa_K2924036">mVenus</a>, it was used to express a protein from cow’s milk, <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> in <i>Synechocystis</i> sp. PCC 6803.
 
===Usage and Biology===
 
===Usage and Biology===
  
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<partinfo>BBa_K2924034 parameters</partinfo>
 
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Revision as of 13:53, 17 October 2019


Pcpc560 + α-s1-casein + T1/T7 Terminator

Strong constitutive cyanobacterial promoter Pcpc560 expressing a-s1-casein with the T1/T7 double terminator.

The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms1. After testing the strength of the promoter with a fluorescent reporter <a href="https://parts.igem.org/part:BBa_K2924036">mVenus</a>, it was used to express a protein from cow’s milk, <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> in Synechocystis sp. PCC 6803.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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