Difference between revisions of "Part:BBa K2984005"
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Revision as of 13:52, 17 October 2019
Level 1 b backbone with GoldenGate Fusionsite
This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 729
Illegal XhoI site found at 721 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal AgeI site found at 1535
Illegal AgeI site found at 1647 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1823
Illegal BsaI.rc site found at 752