Difference between revisions of "Part:BBa K2984005"
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− | The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). | + | <partinfo>BBa_K2984006 short</partinfo> |
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+ | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). | ||
In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases. | In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases. | ||
+ | </html> | ||
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+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K2984006 SequenceAndFeatures</partinfo> |
Revision as of 13:51, 17 October 2019
Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii
This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 298
Illegal NotI site found at 122 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 13
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 401
- 1000COMPATIBLE WITH RFC[1000]