Difference between revisions of "Part:BBa K3185006"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3185006 short</partinfo> | <partinfo>BBa_K3185006 short</partinfo> | ||
| + | ==Usage and Biology== | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| − | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K3185006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3185006 SequenceAndFeatures</partinfo> | ||
| − | + | ==Purification== | |
| + | <br> | ||
| + | <h3><font size="4.5">Expression</font> </h3> | ||
| + | <ul> | ||
| + | <li>Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37<sup>o</sup>C shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer. | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li>Protein was expressed in 0.1mM IPTG for 2hours. | ||
| + | </ul> | ||
| + | <h3><font size="4.5">SDS-PAGE</font></h3> | ||
| + | <br> | ||
| + | <br> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3185006 parameters</partinfo> | <partinfo>BBa_K3185006 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 13:41, 17 October 2019
SPYCatcher -> sfGFP -> LCI KR-2
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1174
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 460
Purification
Expression
- Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37oC shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer.
- Protein was expressed in 0.1mM IPTG for 2hours.
SDS-PAGE