Difference between revisions of "Part:BBa K3185005"

Revision as of 17:32, 4 October 2019 (view source) Registry (Talk | contribs)		Revision as of 13:34, 17 October 2019 (view source) Daidai (Talk | contribs) Newer edit →
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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K3185005 short</partinfo>		<partinfo>BBa_K3185005 short</partinfo>
		+	==Usage and Biology==
−	hoge	+	<br>
−		+	<br>
−	<!-- Add more about the biology of this part here	+
−	===Usage and Biology===	+
		+	<!-- -->
		+	<span class='h3bb'>Sequence and Features</span>
		+	<partinfo>BBa_K3185004 SequenceAndFeatures</partinfo>
		+	==Purification==
		+	<br>
		+	<h3><font size="4.5">Expression</font> </h3>
		+	<ul>
		+	<li>Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37<sup>o</sup>C shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer.
		+	</ul>
		+	<ul>
		+	<li>Protein was expressed in 0.1mM IPTG for 2hours.
		+	</ul>
		+	<h3><font size="4.5">SDS-PAGE</font></h3>
		+	<br>
		+	<br>
	<!-- -->		<!-- -->
	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>

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Revision as of 13:34, 17 October 2019

SPYCatcher -> CenA W68Y

Usage and Biology

Sequence and Features

Purification

Expression

• Cells were grown in 200ml LB media (100μg/ml Ampicillin) at 37^oC shaking at 140 rpm to an OD600 of 0.5, verifying via a spectrophotometer.

• Protein was expressed in 0.1mM IPTG for 2hours.

SDS-PAGE

Sequence and Features